Journal: Materials today. Bio

Article Title: Precise delivery of doxorubicin and imiquimod through pH-responsive tumor microenvironment-active targeting micelles for chemo- and immunotherapy.

doi: 10.1016/j.mtbio.2022.100482

Figure Lengend Snippet: Fig. 10. Immunostaining of tumor tissues in tumor-bearing mice after treatments. (A) Immunohistochemistry images of tumor sections stained with CD3, CD8, and TNF-α antibodies. The scale bar is 100 μm. (B) Immunofluorescence images of tumor tissues after treatments for 12 days. The scale bar is 50 μm. Blue fluorescence represents the cell nucleus stained with DAPI. Green fluorescence represents the iNOS stained with the iNOS antibody conjugated with FITC.

Article Snippet: After 30 min, the tissue slice was stained with diluted iNOS antibody (Miltenyi Biotec, catalog: 130-116-357) at 4 C overnight.

Techniques: Immunostaining, Immunohistochemistry, Staining

Journal: Antioxidants (Basel, Switzerland)

Article Title: Structural Optimization of Cannabidiol as Multifunctional Cosmetic Raw Materials.

doi: 10.3390/antiox12020314

Figure Lengend Snippet: Figure 7. Effects of CBD, S-88745 and S-92153 on the expression of iNOS and COX-2 induced by LPS. In addition to an untreated control group and the LPS−treated group, RAW264.7 cells pretreated with CBD, S-88745 and S-92153 at 0.04, 0.4, and 4 µM for 1.5 h, and then treated all groups with LPS, except the control group for 20 h. The levels of iNOS and COX-2 in the culture supernatants of RAW264.7 macrophages were then detected by western blot. (A) iNOS and COX-2 protein expression levels. (B) Relative ration analysis of iNOS expression. (C) Relative ration analysis of COX-2 expression. The experiments were repeated three times. Each bar illustrates the average ± standard deviation (SD) counted from three experiments. p < 0.05 was considered statistically significant. * p < 0.05 compared to the LPS−treated group; ** p < 0.01 compared to the LPS−treated group; *** p < 0.001 compared to the LPS−treated group; **** p < 0.0001 compared to the LPS−treated group; # p < 0.0001 compared to the control group.

Article Snippet: IL‐6 kits were bought from Elab‐ science (Elabscience, Wuhan, China). iNOS antibody and COX‐2 antibody were pur‐ chased from Signalway Antibody (Signalway Antibody, College Park, MA, USA) CBD and the hit compounds were purchased from TargetMol (TargetMol, Shanghai, China).

Techniques: Expressing, Control, Western Blot, Standard Deviation

Journal: Clinical and Experimental Pharmacology & Physiology

Article Title: Mechanisms of IL‐17A Neutralisation in Alleviating Renal Fibrosis and Inflammation in Spontaneously Hypertensive Rats

doi: 10.1111/1440-1681.70116

Figure Lengend Snippet: Effects of IL‐17A neutralisation on macrophage polarisation in SHR renal tissues. (A) Representative IHC staining of M1 macrophage markers (iNOS and CD86) with quantitative analysis of their positive areas. (B) Representative IHC staining of M2 macrophage markers (Arg‐1 and CD163) with quantitative analysis of their positive areas. (C) Representative immunoblots and relative expression levels of iNOS, CD86, Arg‐1, and CD163 proteins. (D) Proportion of CD86 + and CD163 + cells among CD68 + macrophages. (E) mRNA expression levels of iNOS, CD86, Arg‐1, and CD163. Data are presented as mean ± SD ( n = 6).

Article Snippet: Sections were then incubated overnight at 4°C with primary antibodies against: E‐cadherin (Boster, China), Collagen III (Boster, China), inducible nitric oxide synthase (iNOS) (Boster, China), CD86 (Boster, China), arginase‐1 (Arg‐1) (Boster, China), CD163 (Boster, China), α‐smooth muscle actin (α‐SMA) (Cell Signalling Technology, USA).

Techniques: Immunohistochemistry, Western Blot, Expressing

Journal: CNS Neuroscience & Therapeutics

Article Title: Acetyl‐11‐keto‐beta‐boswellic acid modulates macrophage polarization and Schwann cell migration to accelerate spinal cord injury repair in rats

doi: 10.1111/cns.14642

Figure Lengend Snippet: Information of primary antibodies.

Article Snippet: INOS , 1:200 , Rabbit , bs‐0162R , AB_10855051 , Bioss (Bei‐jing, China).

Techniques: Concentration Assay

Journal: International journal of molecular medicine

Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.

doi: 10.3892/ijmm.2011.659

Figure Lengend Snippet: Figure 5. Immunohistochemical staining of iNOS in rat retinas at 1 day after I/R. (A) sham group; (B) I/R group; (C) control group; (D) fasudil group. The rats in I/R group and control group exhibited a remarkable increase in iNOS reactivity (n=5).

Article Snippet: A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.

Techniques: Immunohistochemical staining, Staining, Control

Journal: International journal of molecular medicine

Article Title: Fasudil, a Rho-associated protein kinase inhibitor, attenuates retinal ischemia and reperfusion injury in rats.

doi: 10.3892/ijmm.2011.659

Figure Lengend Snippet: Figure 6. iNOS mRNA and protein levels in rat retinas at 1 day after I/R were measured by quantitative real-time RT-PCR (A) and Western blot analysis (B), respectively. (A) Compared to the sham group, there was a marked increase in iNOS mRNA in the I/R group and the control group. Fasudil pre-treatment significantly attenuated the increase of iNOS mRNA in retinas of rats with I/R. (B) iNOS protein levels in the above-mentioned groups were consistent with the mRNA expression by Western blotting. Representative blots are shown, and the protein size is expressed in kDa. (n=5, *P<0.05 vs. the sham group, #P<0.01 vs. the control group).

Article Snippet: A rabbit polyclonal antibody against rat iNOS (1:100, Boster Bio-Engineering co. Ltd., Wuhan, china) was used as a primary antibody.

Techniques: Quantitative RT-PCR, Western Blot, Control, Expressing

Journal: mAbs

Article Title: Co-transplantation of human hematopoietic stem cells and human breast cancer cells in NSG mice

doi: 10.4161/mabs.29111

Figure Lengend Snippet: Figure 5. HTM are able to generate human IgM and IgG antibodies. Total IgM (A) and IgG (B) concentration in sera of HTM (BT474 and SK-BR-3) were analyzed by ELISA. Tumor antigen-specific antibody generation in HTM was analyzed on tumor-lysate coated ELISA plates and analyzed in SK-BR-3 transplanted HTM (C) and BT474 transplanted HTM (F). Values are given as arbitrary unit [AI] of optical densities (OD). BT474 transplanted HTM were further subdivided into animals with and without tumor development and the corresponding IgM (D) and IgG (E) concentration for each mouse is displayed. The percentage of mice which did not have detectable tumor growth at the end of the experiments in BT474 transplanted HTM was calculated for all mice dependent on the IgM (G; above or below 20 µg/ml) or IgG concentration (H; above or below 10 µg/ml). (I) Kaplan–Meier survival curves are shown for BT474 transplanted HTM.

Article Snippet: For the detection the following antibodies were used: rabbit anti-human immunoglobulin HRP (SIGMA, A8794), rabbit anti-human IgG (BD Bioscience, 555788), anti-mouse Ig (Cell signaling, 7076S), and anti-biotin HRP [BIORAD, 161–0747)].

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: mAbs

Article Title: Co-transplantation of human hematopoietic stem cells and human breast cancer cells in NSG mice

doi: 10.4161/mabs.29111

Figure Lengend Snippet: Figure 6. HTM generated antibodies that recognize a variety of antigens. BT474 and SK-BR-3 lysates were separated on a SDS-PAGE, transferred to a PVDF membrane and stained with serum of BT474 transplanted (B1–7) and SK-BR-3 transplanted HTM (S1–7), respectively. A polyclonal anti-HER2 antibody was used as a positive control (HER2). Non-humanized tumor mice (TM) served as negative controls (NC). The annotation “St.” refers to the lane of control proteins with standardized size. Tumor-specific antibodies were visualized by anti-Ig detection antibody (A and B) or anti-IgG detection antibody (C). BT474 HTM without detectable tumor are marked with red circles (B2, B3). Tumor cell line specific binding that did not appear on control cell lines are marked with arrows (A and C). (D) Quantification of bands was performed using the Image Quant software package. Each band contributes to the total value by individual thickness and intensity. (E) Western blot units are shown for SK-BR-3 and BT474 HTM divided into animals with or without tumor outgrowth.

Article Snippet: For the detection the following antibodies were used: rabbit anti-human immunoglobulin HRP (SIGMA, A8794), rabbit anti-human IgG (BD Bioscience, 555788), anti-mouse Ig (Cell signaling, 7076S), and anti-biotin HRP [BIORAD, 161–0747)].

Techniques: Generated, SDS Page, Staining, Positive Control, Binding Assay, Software, Western Blot